Medically Reviewed Evidence Based 11 min read·Updated 2 November 2026
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Dubai Health Authority
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EIAC Accredited
ISO 15189:2022
11 min
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Medically Reviewed
Medically reviewed
Nov 2026
Last updated
5 sources
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Key Takeaways
One low testosterone result is not a diagnosis. The Endocrine Society and the American Urological Association both require symptoms plus a low total testosterone confirmed on two separate morning samples.
The Hormones Profile (Male) is AED 159 for total testosterone, LH and FSH, with no fasting required and results in 24 hours. LH and FSH show whether a low result starts in the testes or the pituitary.
When SHBG is unusually high or low, for example with ageing, obesity, diabetes, or thyroid or liver disease, total and free testosterone can disagree. Guidelines prefer free testosterone calculated from total testosterone, SHBG and albumin over direct immunoassays, and we provide the calculation on request.
Book an early morning slot and fast overnight if you can. The British Society for Sexual Medicine advises sampling between 7am and 11am, and home collection starts at 6am.
Search for a testosterone test and you will find two numbers, total and free, plus a third that most people have never heard of, SHBG. Many men test once, get a single result that is a little low or "normal", and are left unsure what it means. The honest answer is that one number from one morning rarely settles anything.
This guide explains what total testosterone, free testosterone and SHBG each measure, how to prepare so your result can be trusted, how doctors decide whether testosterone is truly low, and which test to book.
Important: This article is for general education and does not replace medical advice. Laboratory results should always be interpreted by a licensed physician in the context of your individual history and examination.
Related package
Hormones Profile (Male)
Total testosterone, LH and FSH from one blood sample, to assess testosterone levels and the signals that drive them.
Starting at
159
Start here: one low result does not mean low testosterone
Testosterone is not a fixed number. It follows a daily rhythm and is highest in the morning, it can be lowered by food or glucose, and it can fall during and after an acute illness.1 A sample taken in the afternoon after lunch, or during a week with flu, can read low in a man whose testosterone is adequate.
That is why the major guidelines agree on two points.
Symptoms and blood results must both be present. The Endocrine Society advises diagnosing testosterone deficiency only in men who have symptoms and signs of it and whose testosterone is unequivocally and consistently low.1 The American Urological Association (AUA) also states that the diagnosis is only made when a low total testosterone is combined with symptoms or signs.2
One result is never enough. Both guidelines require a low result to be confirmed with a second morning sample on a separate occasion.1,2 The British Society for Sexual Medicine (BSSM) suggests the two samples are taken preferably four weeks apart.3
The symptoms most closely linked to low testosterone are sexual: fewer morning erections, lower sexual desire and erectile difficulty. In the European Male Ageing Study, a survey of 3,369 men aged 40 to 79, only these three sexual symptoms showed a consistent pattern with low testosterone. Fatigue, low mood and reduced physical vigour were also linked, but they were common in men with normal levels too.4 Tiredness on its own has many causes, and testosterone is only one of them.
The same study found that 2.1% of the men met its definition of late-onset hypogonadism: at least three sexual symptoms together with low total and free testosterone.4 Low testosterone is a real condition, but it is less common than advertising suggests.
Total, free and SHBG: what each number measures
Most of the testosterone in your blood is not free to act. Much of it is tightly bound to a carrier protein called sex hormone binding globulin (SHBG), and most of the rest is loosely bound to albumin. Only a small unbound fraction, the free testosterone, is available to tissues straight away.1
Test
What it measures
When it is most useful
Testosterone, Total
All testosterone: bound to SHBG, bound to albumin, and free
The first test in every guideline, and the basis of diagnosis
Testosterone, Free
Only the unbound fraction
When total testosterone is borderline, or SHBG is likely to be abnormal
Sex Hormone Binding Globulin (SHBG)
The main carrier protein
Explains why total and free can disagree, and is needed to calculate free testosterone
Every guideline starts with total testosterone.1,2,3 Free testosterone and SHBG are second steps, used when total testosterone alone could mislead.
Related test
Testosterone, Total
Total testosterone measures all testosterone in your blood: free, albumin-bound, and SHBG-bound. It is the primary test for evaluating male…
Starting at
65
When the free result matters more than the total
If your SHBG is in the usual range, total testosterone is a fair guide to how much free testosterone you have. The problem comes when SHBG is unusually high or low, because then total and free testosterone can point in different directions.
The Endocrine Society lists common conditions that shift SHBG:1
SHBG tends to be lower with
SHBG tends to be higher with
Obesity
Ageing
Diabetes
Liver disease (cirrhosis, hepatitis)
Underactive thyroid
Overactive thyroid
Glucocorticoid medicines
Some anti-seizure medicines
Androgenic (anabolic) steroids
Oestrogens
Nephrotic syndrome
HIV
Two examples show why this matters. A man with obesity often has low SHBG, so his total testosterone can read low while his free testosterone is adequate. An older man often has higher SHBG, so his total testosterone can look normal while less of it is free.
For this reason, the Endocrine Society recommends measuring free testosterone when total testosterone is at or near the lower limit of normal, or when a condition that alters SHBG is present.1 BSSM gives the same advice, and adds that SHBG should be checked when total testosterone is borderline, especially in older men and men with obesity.3
Direct or calculated free testosterone: the method matters
There are three ways to arrive at a free testosterone number, and they are not equally reliable.
Equilibrium dialysis. The reference method. It is laborious and not widely available, and we do not offer it.
Calculated free testosterone. Worked out from total testosterone, SHBG and albumin using a validated equation. The Endocrine Society accepts a formula that accurately reflects equilibrium dialysis, and BSSM recommends calculated free testosterone.1,3
Direct immunoassay. Measures free testosterone directly in a single test. The Endocrine Society advises clinicians not to use direct analogue-based free testosterone immunoassays because they are inaccurate, and BSSM says analogue displacement immunoassays give unpredictable results.1,3
Where we stand. Our Testosterone, Free test (AED 90) is a direct measurement by chemiluminescent immunoassay (CLIA). We would rather tell you this than let you assume a direct result is the reference standard.
If you or your doctor want calculated free testosterone, ask for it when you book. We will make sure Testosterone, Total (AED 65), SHBG (AED 70) and Albumin (AED 15) are all on your request, so the calculation can be reported. Individual tests booked without a package carry a collection fee when the order comes to under AED 300: AED 100 for home collection, or AED 50 at our lab.
One more limit is worth knowing. The Endocrine Society notes that no harmonised reference range exists for free testosterone, so each laboratory sets its own.1 A free testosterone result is best compared with the range printed on your own report, not with a number from a website.
Related test
Sex Hormone Binding Globulin (SHBG)
SHBG is a protein that binds and carries sex hormones in the blood. Low SHBG increases free testosterone and is associated with PCOS, insulin…
Starting at
70
How to prepare: timing, fasting and what to tell us
Preparation costs nothing and decides whether your result can be trusted.
Book an early morning slot. BSSM advises sampling between 7am and 11am, and says this is particularly important for men under 40.3 Home collection runs from 6am, so a phlebotomist can draw your sample at home inside that window.
Fasting is recommended, not required. Our lab does not require fasting for testosterone. However, food and glucose can lower testosterone, and the Endocrine Society advises a fasting morning sample.1 BSSM agrees that fasting is appropriate for a first test.3 If you can, fast overnight. Plain water is fine.
Avoid testing when you are unwell. The Endocrine Society advises against testing during or while recovering from an acute illness, or while taking short-term medicines such as opioids that suppress testosterone.1 If you take any regular medicine, keep taking it and mention it when you book.
Mention biotin. Biotin can interfere with LH and FSH assays, and the Endocrine Society advises that biotin supplements are stopped for at least 72 hours before testing.1 If you take biotin, tell us and check with your doctor before you pause anything.
Tell us about any hormones you use. If you take testosterone, anabolic steroids or other hormone products, your results reflect what you take, not what your body makes. The reviewing physician needs to know this to read the report correctly.
Repeat in the same lab. The AUA prefers that the two confirming samples are measured in the same laboratory with the same method.2
Related test
Testosterone, Free
Free testosterone measures the biologically active form not bound to proteins. It is a more accurate indicator of androgen status than total…
Starting at
90
Reading a low result: LH and FSH show where the problem starts
Testosterone is made in the testes on instruction from two pituitary hormones, luteinizing hormone (LH) and follicle stimulating hormone (FSH). When testosterone is low, the Endocrine Society recommends measuring LH and FSH to find out which part of that chain is not working.1
Pattern
What it suggests
Low testosterone with high LH and FSH
Primary hypogonadism: the pituitary is signalling, but the testes are not responding
Low testosterone with low or normal LH and FSH
Secondary hypogonadism: the pituitary or hypothalamus is not sending a strong enough signal
A "normal" LH can be the telling result. If testosterone is low, the pituitary would be expected to push LH up. An LH that stays in the normal range is described as inappropriately normal and points toward the secondary pattern.1
In the secondary pattern, the Endocrine Society recommends checking prolactin and iron saturation, with serum ferritin as an alternative way to look for iron overload.1 The AUA also recommends prolactin when total testosterone is low and LH is low or low-normal.2 Both are on our menu: Prolactin (AED 70) and Ferritin (AED 55). A very low total testosterone, below 150 ng/dL (about 5.2 nmol/L), is one of the findings at which both guidelines suggest a doctor consider pituitary imaging.1,2
This is why our Hormones Profile (Male) includes LH and FSH alongside total testosterone. One sample answers both questions: is testosterone low, and if so, where does the problem start.
Different guidelines, different cut-offs
There is no single number that defines low testosterone. Each guideline draws its line slightly differently, and reports may use either nanomoles per litre (nmol/L) or nanograms per decilitre (ng/dL). One nmol/L is about 28.8 ng/dL.
Source
Threshold
Conditions attached
Endocrine Society (2018)
Total testosterone 264 ng/dL (9.2 nmol/L), the lower limit of normal in healthy non-obese young men1
Applies to assays harmonised to the US CDC standard; symptoms and repeat testing still required
AUA (2018)
Total testosterone below 300 ng/dL (about 10.4 nmol/L)2
Two early morning measurements on separate occasions, plus symptoms or signs
European Male Ageing Study (2010)
Total testosterone below 11 nmol/L and free testosterone below 220 pmol/L4
At least three sexual symptoms, in men aged 40 to 79
Supportive evidence only when appropriate symptoms are present
The Endocrine Society also notes that laboratories whose assays are not harmonised to that standard can have reference ranges that vary considerably.1 In practice, your result is read against the reference range on your report, and your doctor weighs it against your symptoms and the guideline that fits your situation.
Related test
Albumin
Albumin is the most abundant protein in blood, produced by the liver. Low levels can indicate liver disease, kidney disease, or malnutrition. A…
Borderline results, suspected SHBG changes, or fertility questions
None of these three needs fasting by our lab's rules, although a fasting morning sample is still recommended for testosterone. Packages carry no collection fee. Total testosterone booked on its own does, so for a home visit the Hormones Profile (Male) at AED 159 costs less than total testosterone alone (AED 65 plus the AED 100 fee).
Total testosterone is also included in the Hair Loss Profile (AED 449), Elite Health Checkup (AED 599), Fitness Profile (AED 599) and Master Health Checkup (AED 899). These do require fasting for some of their markers. They suit a general health review, but a single testosterone value from a checkup still needs a confirming morning sample if it comes back low.
Related package
Male Fertility
A reproductive hormone panel to evaluate the key drivers of male fertility and hormonal balance.
Starting at
399
What these tests cannot tell you
They cannot explain your symptoms on their own. Fatigue, low mood and low sex drive have many causes. A normal testosterone result does not mean nothing is wrong. It moves the question elsewhere.
They are not a screening test for everyone. The Endocrine Society recommends against routine screening of the general population for low testosterone.1 Testing makes most sense when you have symptoms.
Some methods are not on our menu. We do not offer equilibrium dialysis or bioavailable testosterone. If your doctor specifically needs one of these, they will arrange it elsewhere.
Testosterone in women is a different question. Women's levels are much lower and are interpreted in a different context, usually for androgen excess rather than deficiency. Our PCOS blood test guide explains how total testosterone, free testosterone and SHBG are used in women.
Your result is back: what now?
Take it to a doctor. Every report is physician-reviewed, but a diagnosis also depends on your symptoms, your history and an examination.
Normal result and no symptoms: there is usually no reason to keep retesting.
Low result once: arrange a second early morning sample on a separate day, ideally in the same lab, before anyone draws a conclusion.1,2,3
Borderline result: ask your doctor whether SHBG or calculated free testosterone would help, particularly if you are older, carry extra weight, or have diabetes, thyroid or liver disease.
Low twice, with symptoms: your doctor will use LH and FSH to look for the cause, and may add prolactin, ferritin or other tests.
If fertility matters to you: the AUA recommends a reproductive health evaluation before any treatment in men with testosterone deficiency who are interested in fertility.2 Say so early.
If you already take testosterone or anabolic steroids: your doctor decides when and how to test. Do not start, stop or change anything based on a single result.
Book a testosterone test
Total testosterone, LH and FSH from one early morning sample, collected at home anywhere in Dubai from 6am, with a physician-reviewed report in 24 hours. Book the Hormones Profile (Male), or ask a clinician first if you are not sure which test suits you.
This article is for general education and is not a substitute for medical advice. Low testosterone cannot be diagnosed from a single blood test, and you should not start, stop or change testosterone, anabolic steroids or any other medicine on the basis of this page. Intel Lab Diagnostics is licensed by the Dubai Health Authority (licence 0046381) and accredited by EIAC to ISO 15189:2022. Prices and turnaround times were verified on 2 November 2026.
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Health package
Hormones Profile (Male)
Total testosterone, LH and FSH from one blood sample, to assess testosterone levels and the signals that drive them.
From 159
Health package
Male Fertility
A reproductive hormone panel to evaluate the key drivers of male fertility and hormonal balance.
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Blood test
Testosterone, Total
Total testosterone measures all testosterone in your blood: free, albumin-bound, and SHBG-bound. It is the primary test for evaluating male…
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SHBG is a protein that binds and carries sex hormones in the blood. Low SHBG increases free testosterone and is associated with PCOS, insulin…
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FAQ
Frequently Asked Questions
Common questions on this topic, answered by our lab team. Can't find yours?
At Intel Lab, total testosterone on its own is AED 65. Booked without a package, it carries a collection fee when the order comes to under AED 300: AED 100 for home collection, or AED 50 at our lab. The Hormones Profile (Male), which adds LH and FSH, is AED 159. The Male Fertility package, which includes total and free testosterone, SHBG and six other hormones, is AED 399. Packages carry no collection fee, at home or at our lab.
Start with total testosterone. Every major guideline uses it as the first test. Free testosterone becomes important when total testosterone is at or near the lower limit of normal, or when a condition that changes SHBG is present, such as obesity, diabetes, ageing, or thyroid or liver disease.
The Endocrine Society advises against direct analogue-based free testosterone immunoassays because they are inaccurate, and prefers equilibrium dialysis or a calculation from total testosterone, SHBG and albumin. Our free testosterone test is a direct immunoassay (CLIA). If you or your doctor want calculated free testosterone, ask when you book and we will make sure total testosterone, SHBG and albumin are all on your request.
Our lab does not require fasting for testosterone, but fasting is recommended. Food and glucose can lower testosterone, so the Endocrine Society advises a fasting morning sample. Plain water is fine.
Early morning, when testosterone is highest. The British Society for Sexual Medicine advises sampling between 7am and 11am, which matters most for men under 40. Home collection starts at 6am.
Not from one result. The guidelines require symptoms of testosterone deficiency and a low result confirmed on a second morning sample on a separate day. A doctor then uses LH and FSH to work out where the problem starts.
Total testosterone and the Hormones Profile (Male) are reported in 24 hours. The Male Fertility package takes 48 hours. Results arrive as a physician-reviewed digital report.
References & Sources
This article cites peer-reviewed research and guidance from recognised medical authorities.
Specialisation: Clinical PathologyDHA Reg. No. 65077850-00725+ years experience
Dr Nirupama Sabhapathy is a DHA-licensed Specialist in Clinical Pathology and Lab Director at Intel Lab, Dubai. She oversees laboratory operations and quality assurance across every testing panel, and each abnormal result passes a specialist review before it reaches you.
Dr Nirupama Sabhapathy is a DHA-licensed Specialist in Clinical Pathology and Lab Director at Intel Lab, Dubai. She trained at the Manipal Academy of Higher Education and holds the DNB in Pathology from the National Board of Examinations, New Delhi.